Naïve CD4 T cell (CD62L+CD44-) were isolated from spleens of normal WT mice (10 week old), cultured in Th0, Th1, Th2, Th17 and iTreg conditions and Lcn2 mRNA quantified on day 4 using real-time PCR. Lcn2 mRNA expression in Th1, Th2 and Th17 cells was significantly (p< 0.001) higher compared to Th0 cells. Among differentiated CD4 T cells, Th17 cells had most significant increase in Lcn2 expression followed by Th1 and Th2 cells. Lcn2 expression in iTreg cells was not detectable. Data presented as mean±SE.***P < 0.001.