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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: J Immunol. 2019 Dec 20;204(3):644–659. doi: 10.4049/jimmunol.1901211

Figure 7. IL-10 neutralization leads to increased collagenization and fibrosis in lung granulomas.

Figure 7.

A) Anti-IL-10 treated animals have more fibrosis in lung granulomas compared to control animals at the time of necropsy (both 4 and 8 weeks of infection), using a semi-quantitative assessment by a pathologist (EK). Fisher’s exact test p-value was reported. B) Lung granulomas from anti-IL-10 treated animals have more collagen at 8 weeks post- Mtb infection than control animals at the time of necropsy, as quantified by mean pixel intensity of blue from Masson’s trichrome staining of a random subset of granulomas. Each point represents a granuloma, each color is a NHP (gray indicates historical control). Lines at medians. Kruskal-Wallis Test was performed and Dunn’s Multiple Comparisons adjusted p-values were reported. C) Representative granulomas from each treatment group at each time post-infection (4 and 8 weeks) were stained for collagen by Masson’s trichrome staining, fibroblast (vimentin), macrophage (CD68), myofibroblast (α-SMA) markers, collagen and macrophage (CD163, CD68) markers, IL-4/IL-13 signaling (pSTAT6) and myeloid (CD11c) markers, and TGF-β signaling (pSMAD3) and myeloid (CD11c) markers as labeled in the figure. White boxes show inset of the area boxed in the granuloma for more detail of representative area with collagen, scale bars in trichrome images represent 500μm.