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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: J Immunol. 2019 Dec 23;204(3):510–517. doi: 10.4049/jimmunol.1901357

Figure 1:

Figure 1:

IL-27p28 is made by cDC1s in the draining node following peripheral subunit immunization. (A-E) IL-27p28-eGFP mice were immunized in the footpad (A-C) or footpad, ear or thigh muscle (D, E) with 10ug polyIC and 25ug OVA, and dLNs harvested 6–12 hours later. (A) Representative gating strategy identifying cDC1s and demonstrating GFP signal following immunization. (B, C) cDC1s, cDC2s, monocytes, B cells and T cells were identified in order to determine IL-27p28-eGFP production by each cell type. (D, E) the GFP signal from cDC1s was measured in the cervical, popliteal and inguinal dLNs following immunization between the ear layers, in the footpad or in the thigh respectively. Data indicate means ± SEM, n ≥ 3 mice per group, representative of 2 or more experiments. **p < 0.01, ****p < 0.0001.