Figure 2:

cDC1 IL-27p28 production in the dPLN peaks at 12 hours after immunization. (A-H) C57BL/6 (A, B), C57BL/6-Ccr7^tm1.1Dnc/J (C, D) or IL-27p28-eGFP (E-H) mice were immunized in the footpad with 10ug polyIC and 25ug OVA and dPLNs harvested and examined by flow cytometry at the indicated time point. (A, B) Gated on total DCs. (C-H) Gated on total DCs or as indicated. Data indicate means ± SEM, n ≥ 3 mice per group, representative of 2 or more experiments. **p < 0.01, ****p < 0.0001.