Figure 3:

cDC1 IL-27p28 production at 12 hours in the dPLN correlates with systemic T cell counts at 7 days. (A-C) IL-27p28-eGFP mice were immunized in the footpad with 10ug indicated adjuvant and 25ug OVA and dPLNs harvested 12 and 24 hours later. Gated on cDC1s. (D, E) C57BL/6 mice were immunized in the footpad with 10ug indicated adjuvant and 25ug OVA and dPLNs, blood and spleens were harvested 7 days later. Gated on CD8s. (F) Linear regressions of correlations between data in Fig. 3 C and Fig. 3 E. Data indicate means ± SEM, n ≥ 3 mice per group, representative of 2 or more experiments.