Figure 4:

cDC1 IL-27p28 production at 12 hours correlates with protection against infection at memory time points. (A, B) C57BL/6 mice were immunized in the footpad with 10ug indicated adjuvant and 25ug OVA and dPLNs, blood and spleens were harvested 10 days later (data from spleen shown). (C) Linear regression of correlation between data in Fig. 3 C and Fig. 4 B. (D) C57BL/6 mice were immunized in the footpad with 10ug indicated adjuvant and 25ug OVA and spleens were harvested 50–80 days later. (E) Linear regression of correlation between data in Fig. 3 C and Fig. 4 D. (F) Memory stage mice from Fig. 4 D were challenged intravenously with 1.5×10⁵ LM, and 4 days later livers were harvested, homogenized and plated in serial dilution on BHI plates containing erythromycin. CFU of LM obtained per gram of liver. (G) Nonlinear regression of correlation between data in Fig. 3 C and Fig. 4 F. Data indicate means ± SEM, n ≥ 3 mice per group, representative of 2 or more experiments.