Figure 5:

Early IL-27p28 production predicts the efficacy of novel adjuvants in generating protective T cell responses. (A) IL-27p28-eGFP mice were immunized in the footpad with indicated amount of polyIC and 25ug OVA and dPLNs harvested 12 later. (B) C57BL/6 mice were immunized in the footpad with indicated amount of polyIC and 25ug OVA and spleens were harvested 10 days later. (C) Linear regression of correlation between data in Fig. 5 A and Fig. 5 B. (D) IL-27p28-eGFP mice were immunized in the footpad with 10ug adjuvant and 25ug OVA and dPLNs harvested 12 hours later. (E) gMFIs for previously unexamined adjuvants from Fig. 5 D were normalized against gMFIs obtained from polyIC-immunized mice harvested and analyzed simultaneously, and these adjusted gMFI levels were used to calculate predicted T cell numbers (grey boxes) using the linear regression formula from Fig. 4 C. Experimental values (black circles) were obtained by immunizing C57BL/6 mice in the footpad with 10ug indicated adjuvant and 25ug OVA and harvesting spleens 10 days later. (F) Overlay of values obtained in Fig. 5 D and 5 E with correlation plot from Fig. 4 C. Data indicate means ± SEM, n ≥ 3 mice per group, representative of 2 or more experiments.