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. Author manuscript; available in PMC: 2021 Jan 22.
Published in final edited form as: Neuron. 2019 Dec 3;105(2):322–333.e5. doi: 10.1016/j.neuron.2019.10.028

Figure 6. Role of pCd neurons in a female-induced enhancement of male aggressiveness.

Figure 6.

(A) Schematic illustrating female induced inter-male aggression experiment. Single-housed male flies were pre-incubated in vial with or without (control) a virgin female for 5 min. Subsequently, pairs of pre-incubated males were placed in behavioral arenas with an agarose substrate. (B) Lunge frequency per fly after pre-incubation without (white) or with (blue) a female. n=32 flies each. Statistical test used was a Mann-Whitney U-test. **** P < 0.001. (C) Schematic of experimental design. (D) Lunge number before (pre-stim.) and after (post-stim.) GtACR1-mediated neural silencing. Green lines depict exposure to green light (530 nm, 10 Hz, 10 ms pulse-width) for 10 s. Gray points show lunge frequencies for individual flies, and black points show mean values. Statistical tests used were Wilcoxon signed test (within fly comparison) and Kruskal-Wallis test (between genotype comparison). ** Dunn’s corrected P < 0.01, **** P < 0.0001