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. 2020 Jan 24;40(1):BSR20193489. doi: 10.1042/BSR20193489

Figure 4. SOX4 worked as a functional target of miR-129-1-3p in NSCLC.

Figure 4

(A) The candidate miRNAs expressions were detected by RT-qPCR assays in cells transfected with miR-129-1-3p mimics. (B) Bioinformatics predicted putative binding sites between miR-1291-1-3p and SOX4. (C) SOX4 expression and protein were detected in cells transfected with miR-129-1-3p mimics. (D) RIP assays were conducted to confirm miR-129-1-3p and SOX4 coexisted in RISCs. (E) The overexpressing efficiency of pcDNA3.1/NORAD was evaluated by RT-qPCR assay. (F) RNA pull-down assays were conducted to investigate the effects of NORAD overexpression on the interaction between miR-129-1-3p and SOX4 in H446/DDP and A549/DDP cells. (G) Luciferase reporter assays were conducted to verify the combination between miR-129-1-3p and SOX4; *P < 0.05, **P < 0.01.