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. 2020 Jan 24;40(1):BSR20191043. doi: 10.1042/BSR20191043

Figure 5. HRS protected intestinal epithelial cell from HR injury through down-regulating miR-199a-3p expression and reactivating mTOR-related pro-survival pathway.

Figure 5

(A) Relative miR-199a-3p expression was measured after HR with or without HRS treatment or transfecting with miR-199a-3p mimics or inhibitor in intestinal epithelial cell IEC-6. Cellular viability of IEC-6 was detected using CCK-8 method (B), and mRNA expression of IGF1, PIK3r1 and mTOR (C) were detected after HR with or without HRS or transfecting with miR-199a-3p mimics or inhibitor in IEC-6; n = 6. *P < 0.05. HR: hypoxia for 2 h/reoxygenation for 60 min; HR+negative con: miR- 199a-3p negative control was transfected to IEC-6 24 h before HR performed; HR+inhibitor: miR-199a-3p inhibitor was transfected to IEC-6 24 h before HR performed; HR+HRS: IEC-6 cells were pretreatment with HRS medium for 24 h before cells subjected to hypoxia/reoxygenation; HR+HRS+mimics: miR-199a-3p mimics was transfected to IEC-6 24 h before HRS treatment and subsequent HR performed.