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. 2020 Jan 24;10:1130. doi: 10.1038/s41598-020-58002-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FACS comparison of VSD4-NavAb nanodiscs to VSD4-NavAb proteoliposomes in binding to hybridoma. 141B8, a hybridoma previously shown to produce antibodies that bind to VSD4-NavAb proteoliposomes, was used to investigate whether antigen-incorporated nanodiscs versus proteoliposomes could be used in FACS to identify antigen-binding hybridoma. VSD4-NavAb nanodiscs (A) or proteoliposomes (B) were biotinylated and added to the 141B8 hybridoma cells at the indicated amounts with Alexa Fluor 647-conjugated streptavidin added subsequently for detection (x-axis). Alexa Fluor 488-conjugated goat anti-mouse IgG was used to confirm surface expression of IgG on the hybridoma (y-axis). The population in quadrant 2 (Q2) represents 141B8 hybridoma expressing IgG that bind to VSD4-NavAb nanodiscs (A) or proteoliposomes (B).