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. 2020 Jan 24;10:1130. doi: 10.1038/s41598-020-58002-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Single cell sorting of hybridoma using VSD4-NavAb nanodiscs enriches for hybridoma producing antibody to VSD4-NavAb. (A) Shown is an ELISA using VSD4-NavAb nanodisc coated plates to examine binding of antibodies collected in the media from the single cell sorted hybridoma. (B) ELISA demonstrating that antibodies shown to bind to VSD4-NavAb nanodiscs also bind to VSD4-NavAb proteoliposomes. (C) Antibodies that bound to VSD4-NavAb proteoliposomes bound to VSD2-NavAb proteoliposomes by ELISA.