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. 2020 Jan 24;10:1143. doi: 10.1038/s41598-020-57560-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PDD005 modulates tau phosphorylation, IL-1β, GSK-3β in organotypic hippocampal slice cultures (OHSCs)-from 3x Tg- AD mice. (A) Graph illustrating the effect of PDD005-mediated attenuation of IL-1β in OHSCs from transgenic tau mice (3xTg-AD). Transcriptional expression of IL-1β was quantified by RT-PCR. Data expressed as the relative expression normalized to the housekeeping gene HPRT (2^−ΔCt). Significant differences determined by using one-way ANOVA with Tukey’s test. *P < 0.05 and ***P < 0.001 indicate significant differences between PDD005 and the vehicle group. Data expressed as means ± SEM with n = 2–6 inserts/condition and 10 slices/insert. (B,C) Representative immunoblot of the effect of PDD005-mediated attenuation of tau phosphorylation (Thr 212) in OHSCs-3x Tg- AD mice and densitometric analysis of intensity of immunoblots. Data expressed as means ± SD with n = 2 inserts/condition and 10 slices/insert. Significant differences determined by using Student’s t test.*P < 0.05 indicates significant differences between PDD005 and the vehicle group. (D,E) Representative immunoblot of the effect of PDD005-mediated phosphorylation of GSK-3β in OHSCs in 3x Tg- AD mice (D) and densitometric analysis of intensity of immunoblots (E). Each bar is expressed as mean ± SEM (n = 4 mice for all groups). Significant differences determined by using one-way ANOVA with Tukey’s test. ns = not significant *P < 0.05 compared with vehicle group.