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. 2020 Jan 24;11:518. doi: 10.1038/s41467-020-14313-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a MHZ1 expression induced by ethylene is dependent on OsEIN2 and OsEIL1. Two-day-old etiolated seedlings of WT or Osein2 and Oseil1 were treated with 10 ppm ethylene or air. Data are means ± SD, n = 4. b Binding of OsEIL1 protein to the OsEIL1 binding site (EBS) containing region of MHZ1 promoter in vitro. GST-tagged OsEIL1 N-terminus protein was isolated and incubated with biotin-labeled probes carrying EBSs of MHZ1 promoter. An excess of unlabeled probe was added as the competitor. c Transient expression of OsEIL1 in tobacco leaves activated MHZ1 promoter activity. Relative luminescence intensity was calculated, data are means ± SD from three biological replicates. Source data are provided as a Source Data file.