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. 2020 Jan 24;11:480. doi: 10.1038/s41467-019-13918-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic representation of the sequence of mouse behavioral experiments. b Y-maze percentage alternation analysis of WT (n = 23), Kcnk9KO^mat (n = 27), and Kcnk9KO^hom (n = 44) mice. A spontaneous alternation was defined as consecutive entries into all three arms without revisiting an arm. Kcnk9KO mice display a significant decrease in percentage alteration compared with WT mice. One-way ANOVA: F(2, 91) = 7.261, P = 0.0012; followed by Bonferroni’s multiple comparison post hoc test, *P < 0.05, **P < 0.01. c Total locomotor activity (distance traveled in the home cage) in light (12 h, sun symbol)/dark (12 h, moon symbol) phase. The left section shows no difference in distance traveled in the light phase of WT (n = 13), Kcnk9KO^mat (n = 10) and Kcnk9KO^hom (n = 9) mice. One-way ANOVA: F(2, 29) = 2.281, P = 0.1203. The right section depicts the nocturnal activity of WT (n = 18), Kcnk9KO^mat (n = 13) and Kcnk9KO^hom (n = 15) mice. Kcnk9KO^mat and Kcnk9KO^hom mice display significantly increased nocturnal hyperactivity compared with WT littermates with activity scores of Kcnk9KO^mat mice intermediate between Kcnk9KO^hom and WT mice. One-way ANOVA: F(2, 43) = 22.70, P < 0.0001; followed by Bonferroni’s multiple comparison post hoc test, *P < 0.05, **P < 0.01, ****P < 0.0001. b, c Behavioral experiments were performed in seven independent sessions. d Non-canonical Kcnk9 imprinting in (C57BL/6xCast/Ei)F1 hybrid mice. Quantification of Allele-Specific Expression by Pyrosequencing (QUASEP) of several brain regions from (C57BL/6xCast/Ei)F1 hybrid mice; maternal allele (red) and paternal allele (blue). Cerebellum n = 14, pons n = 10, olfactory bulb n = 12, cortex n = 14, hippocampus n = 14, hypothalamus n = 9, striatum n = 5, midbrain n = 5, medulla n = 5, and locus coeruleus n = 4; n = biologically independent samples. a–d Data are means ± 1 SEM (standard error of the mean). Statistical analyses and approaches are provided in Supplementary Table 1. Source data are provided as a Source Data file. The mouse images in this figure were created using Servier Medical Art templates, which are licensed under a Creative Commons Attribution 3.0 Unported License; https://smart.servier.com.