Figure 10.

Effect of normoxia and hypoxia on the invasion of ER−ve breast cancer cells through either BME alone or a double layer of ER + cells and BME. Untreated pII cells were loaded into the top chamber of a Cultrex BME cell invasion plate which was previously loaded with BME only. pII cells [treated with vehicle (C) or CoCl₂, 100 µM] were loaded into the top chamber of a Cultrex BME cell invasion plate which was previously loaded with another layer of EII cells above the BME. pII cells invading into the lower chamber, towards serum, were detected as described in Methods. Histobars represents mean ± SEM of 3 independent determinations. It should be noted that no EII invasion towards the lower chamber was observed. A schematic diagram explaining each experimental setup is shown on the top of the figure.