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. 2020 Jan 24;10:1127. doi: 10.1038/s41598-020-58055-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of CoCl₂ or DFO treatment on HIF-1α localization in ER−ve breast cancer cells. pII cells were seeded in 8-chamber Ibid slides and allowed to grow for 48 h at 37 °C/ 5% CO₂. Cells were either untreated (control) or treated with (A) CoCl₂ or (B) DFO (100 µM) for times indicated. Cells were then fixed and stained with HIF1α antibody (red), phalloidin (green, to stain F-actin cytoskeleton), and DAPI (blue, to stain the nucleus), 20x magnification. Scale bar represents 20 µm.