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. 2020 Jan 24;10:1134. doi: 10.1038/s41598-020-58113-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological staining of explanted anastomoses: (a) Green trichrome: Control group; (b) Green trichrome: PCL group, the empty space on the site of application of the nanomaterial can be seen in the upper layer, surrounded by normal granulation tissue; (c) Green trichrome: PLCL group, a much thinner empty area can be seen in the upper layer, also surrounded by normal granulation tissue; (d) PSR staining, collagen fibres stained yellow, stereological mesh; (e) vWF factor staining, the endothelial cells stained brown, stereological grid; (f) MAC 387 staining stereology, positive cells stained blue, stereological grid; (g) magnification of vWF staining stereology with a positive cross in the upper right corner.