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. 2020 Jan 24;11:498. doi: 10.1038/s41467-020-14374-1

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© The Author(s) 2020, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Undifferentiated pleomorphic sarcoma (UPS) tumours are generated by injection of adenovirus expressing Cre-recombinase (AdCre) into hindlimb muscles of LSL-Kras^G12D/+;Trp53^fl/fl (KP) and LSL-Kras^G12D/+;Trp53^fl/fl;Epas1^fl/fl (KPH2) mice, and harvested 8–10 weeks after viral delivery. b Tumour weights of KP (n = 8) and KPH2 tumours (n = 7) at 9 weeks post-AdCre injection. Error bars are ± s.d; **p < 0.005. c MetaboAnalyst pathway enrichment analysis of VIP > 1 metabolites in muscle (Mus.) and KPH2 comparison following LC/MS; *p < 0.05. N = 4 with three technical replicates. d Normalized ion counts for glutamine, glutamate, aspartate, and asparagine (left to right) in gastrocnemius muscle (gastroc. muscle), KP, and KPH2 tumours. N = 4 with three technical replicates. Data represent mean ± s.d; *p < 0.05, **p < 0.005, ***p < 0.0005. e Normalized ion counts for glutamine, glutamate, aspartate, and asparagine (left to right) in gastroc. muscle, KP, and LSL-Kras^G12D/+;Trp53^fl/fl;Arnt^fl/fl (KPA) tumours. N = 4 with three technical replicates; *p < 0.05, **p < 0.005. f Proliferation of murine mesenchymal stem cells (MMSC; top left), murine myoblasts (C2C12; top right), KP tumour-derived cells (KP-6634; bottom left), and KPH2 tumour-derived cells (KPH2-7215; bottom right) grown in media with or without glucose (Gluc) and/or glutamine (Q). N = 3 with three technical replicates each. Data represent mean ± s.e.m.; **p < 0.005, ***p < 0.0005. g Viability of MMSCs, C2C12s, differentiated C2C12s (3 days, C2C12 D3; 6 days, C2C12 D6), KP-6634s, and KPH2-7215s grown in media with or without Q and assessed after 48 h. N = 3 with two technical replicates. Data represent mean ± s.e.m; **p < 0.005, ***p < 0.0005. p-Values were calculated from a two-tailed Student’s t-test for b, d–g. Source data are provided as a Source Data file.