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. 2020 Jan 24;11:498. doi: 10.1038/s41467-020-14374-1

Fig. 3. Glutamine anaplerosis promotes nucleoside production in UPS tumour cells.

Fig. 3

a Schematic depicting purine and pyrimidine biosynthesis. b Normalized ion counts for carbamoyl phosphate (carbamoyl-P), orotate, adenine, and guanine (left to right) in gastrocnemius muscle (gastroc. muscle), KP, and KPH2 tumours. N = 4 with three technical replicates. Data represent mean ± s.d.; *p < 0.05, ***p < 0.0005. c Concentration of total adenine levels in C2C12s, KP-6634s, and KPH2-7215s. N = 3 with two technical replicates. Data represent mean ± s.e.m.; *p < 0.05. d Mass isotopomer analysis of M + 2 and M + 3 enrichment in adenine, represented as atoms percent excess (APE), in C2C12s, KP-6634s, and KPH2-7215s cultured for 5 h with 15N2-glutamine. N = 3 with two technical replicates. Data represent mean ± s.e.m. e Growth of C2C12s, KP-6634s, and KPH2-7215s (left to right) grown in media with 0–2 mM glutamine (Q) and supplemented with 50 μM adenosine, guanosine, cytidine, and thymidine (Nucleosides) for 72 h. N = 3 with three technical replicates. Data represent mean ± s.e.m.; **p < 0.005, ***p < 0.0005. p-Values were calculated from a two-tailed Student’s t-test for b–e. Source data are provided as a Source Data file.