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. 2020 Jan 24;10:1149. doi: 10.1038/s41598-019-57387-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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UHPLC-MS analysis of MPCs and RSCs H₂O extracts. (A) Base peak chromatogram of MPCs H₂O extract. (B) Base peak chromatogram of RSCs H₂O extract depicting the tentative identification of previously unreported sulphate derivatives of spinochromes and suspected previously reported spinochromes. I: Sulphate derivative of spinochrome E isomer 1. II: Sulphate derivative of spinochrome E isomer 2. III: Suspected sulphate derivative of spinochrome D. IV: Suspected sulphate derivative of spinochrome dimer (m/z 614.9926). V: Suspected sulphate derivative of spinochrome C. VI: Suspected sulphate derivative of spinochrome related dimer ethylidene-6, 60-bis (2,3,7-trihydroxynaphthazarin) (m/z 580.9871). All tentatively identified metabolites were absent in extracts of the MPCs substrate, with the exception of trace amounts of sul-SpinD. Scale bar 50 µm.