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. 2020 Jan 24;10:1100. doi: 10.1038/s41598-020-57461-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Callus development over the course of the study for both 0.85 mm and 1.45 mm defects. Two thresholds are shown for each mouse, most significant is the difference between observed state of healing at week 2, where for the lower threshold both groups are fused and for the higher threshold no healing has occurred.