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. 2020 Jan 24;10:1163. doi: 10.1038/s41598-020-58305-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of interaction between recombinant ligands (rDIII and rNadA) and protein extract of hBMECs using semi-quantitative ELISA. A – rDIII; B - rNadA. Interaction was detected with HisProbe-HRP conjugate. Framed reagents were coated into microtiter wells. Data present means of triplicates with ± S.D. CB – coating buffer; hBMECs – protein extract of human brain microvascular endothelial cells; rDIII – recombinant DIII; rNadA – recombinant NadA. Please note that all wells were blocked with blocking buffer after overnight coating. Interaction was detected with HisProbe-HRP conjugate.