Fig. 4. Kindlin-2 loss impairs pancreatic islet development.

a–d Immunofluorescence (IF) staining. E13.5, E15.5, E18.5, P0, and P7 pancreatic sections of control and K2-RIP mice were subjected to IF staining using antibodies against insulin (for β-cell), glucagon (for α-cell), somatostatin (for δ-cell), or DAPI (not shown). Pictures were captured (a) and the total number of α-cells (red), β-cells (green), and δ-cells (blue) counted and expressed as percentages of total cells in islets (b–d). We measured the islet cell composition from 22 to 34 islets per mouse. We analyzed islets from two sides (each slide had 3–5 pancreatic sections on it) from each mouse and obtained an average value from each slide. Thus, we obtained two data points for each mouse. For statistical analysis, we used the average value of those two data points for each mouse. N = 5 mice per genotype at all time points. Scale bar, 50 μm. *P < 0.05, versus control, Student’s t test. Because islets were not completely formed in E13.5 and E15.5 mouse pancreatic tissue, we were unable to accurately define the borders of the islets and did not determine the islet composition at those two time points. Results are expressed as mean ± standard deviation. Source data for b–d are provided as a Source Data file.