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. 2020 Jan 24;11:479. doi: 10.1038/s41467-019-14169-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a–f The transmission electron micrographs of the notochord in WT (a, d) and dstyk mutant (b, c, e and f) at 48 hpf. Note that the vacuoles were smaller in the dstyk mutant, and there were many scatteredly distributed small vesicles that were wrapped by single layer membrane in the cytoplasm of dstyk mutant. g, h The ultrastructure of the notochord sheath in the WT (g) and dstyk mutant (h). Note the straight, well-organized sheath layer in the WT and wavy, disordered organization of the medial layer in the dstyk mutant. i Ultrastructure of the notochord showed not well vacuolated epithelia-like cells (white arrow) aggregated in dstyk mutant. f, g and h were magnified image of the dotted boxes in c, d and e, respectively. Black arrowheads indicate many small vesicles in the mutant cytoplasm. All images are shown longitudinal sections. The scale bars represent (a–c, and i) 5 µm, (d–f) 1 µm and (g, h) 500 nm. va, vacuole; s, notochord sheath; sc, sheath cell; pm, plasma membrane; vm, vacuole membrane; i, inner laminin-rich layer, m, medial layer, and o, outer collagen-rich layers of the notochord sheath.