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. 2020 Jan 24;11(1):63. doi: 10.1038/s41419-020-2227-4

Fig. 4. α-MGT inhibits dimerization and nuclear translocation of STAT3 and expression of STAT3 target genes in HCC cells.

Fig. 4

a HepG2 cells transfected with Flag- and GFP-tagged STAT3 plasmids for 24 h. Next, cells were treated with α-MGT for 6 h, followed by incubation with IL-6 (10 ng/mL) for 30 min, then subjected to immunoprecipitation, and immunoblotted with Flag or GFP antibody (Ab). Whole cell extracts were processed for western blotting with the indicated antibodies. b HepG2 cells were pre-treated with α-MGT for 6 h, followed by stimulating with IL-6 (10 ng/mL) for 30 min. Anti-STAT3 antibody (red) was used to locate STAT3 in cells. Cell nuclei were stained with DAPI. Bar = 10 μm. c, d HepG2 and SK-Hep-1 cells were treated with α-MGT for 24 h. The mRNA levels of Bcl2, survivin, cyclin D1, and c-Myc were measured by RT-qPCR. β-actin was used as an internal control. e After treatment of α-MGT for 24 h, the protein expression of Bcl2, survivin, cyclin D1, and c-Myc were analyzed using western blotting. Data are expressed as mean ± SD, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 vs. vehicle control.