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. 2020 Jan 24;11(1):63. doi: 10.1038/s41419-020-2227-4

Fig. 6. SHP1 is important for α-MGT-mediated actions in HCC cells.

Fig. 6

a, b The expression of SHP1 and SHP2 were detected in α-MGT- or vehicle-treated HepG2 or SK-Hep-1 cells by western blotting assay. c HepG2 cells were treated with α-MGT for the indicated times. The expression of SHP1 was measured using western blotting. β-actin was used as the loading control. HepG2 cells were transfected with SHP-1 siRNA or scrambled siRNA. After 24 h, cells were treated with α-MGT (20 μM) for additional 24 h. Next, cells were lysed and applied to immunoblotting with the indicated antibodies (d), or measured by SRB staining (e). f HepG2 cells were pre-treated with α-MGT (20 μM) for 6 h, and then exposed to CHX (50 μg/mL) for additional 9 h. The protein level of SHP1 was detected by western blotting. g HepG2 cells were pre-treated with MG132 (10 μM) for 6 h, and followed by incubation with α-MGT (20 μM) for 9 h. The cell lysates were then subjected to immunoprecipitation using IgG or SHP1 antibody, and immunoblotted with the indicated antibodies. Data are expressed as mean ± SD, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 vs. control.