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. 2020 Jan 24;10:1141. doi: 10.1038/s41598-020-57964-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Principal component analysis (PCA) was performed on samples after removal of lowly expressed miRNAs (Ct >25) and normalisation of Ct values. (B) Correlation plots showing the correlation between sex, genotype (GT), age, BAL Total cell count (BAL), neutrophil elastase levels (NEL), IL-8 concentration (IL-8), Haemophilus spp. (Haem), Escherichia spp. (Esch), Staphyloccus spp. (Staph), antibiotic treatment (Antib), PC1 and PC2. The size of the circles is proportional to the strength of the correlation and the colour represents the direction i.e. a large dark blue circle represents a strong positive correlation and a large dark red circle represents a strong negative correlation.