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. 2020 Jan 24;11:468. doi: 10.1038/s41467-019-14148-4

Fig. 5. SFB+ microbiota is sufficient for low calcium diet to expand intestinal and BM Th17 cells.

Fig. 5

a–d Frequency of PP and BM Th17 cells and small intestine (SI) and BM levels of Il17a transcripts in SFB+ TAC and JAX and SFB- JAX mice treated with control diet or low calcium diet for 4 weeks with antibiotics (Abx) or without antibiotics (No Abx), a, b n = 9–10 mice per group; c, d n = 5 mice per group. e, f Frequency of PP and BM Th17 cells in GF mice, SFB monoassociated GF mice, and GF mice colonized with JAX mice microbiota ±SFB treated with control diet or low calcium diet for 4 weeks, n = 5–10 mice per group. Data are expressed as Mean ± SEM. All data were normally distributed according to the Shapiro-Wilk normality test. Data in a–d were analyzed by two-way analysis-of-variance and post hoc tests applying the Bonferroni correction for multiple comparisons. Data in panels e,f were analyzed by unpaired t-tests. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 compared to the indicated group. Source data are provided as a Source Data file.