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. 2020 Jan 24;10:1110. doi: 10.1038/s41598-020-57969-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neutrophils do not restrict viral replication during RSV infection. (a) C57BL/6 or wt mice were mock (PBS) or RSV infected for 18 h, 4 and 8 days. To deplete neutrophils, 150 µg i.p. α-Ly6G or isotype control antibody was administered on days −1, 1, 3, 5 and 7. (b) RSV L gene copy number was quantified in lung tissue by RT-qPCR. Copy numbers were determined using a plasmid standard and the results were normalized to Gapdh levels. (c) Infectious viral particles in the lung on day 4 were quantified by immunoplaque assay. Data from (b) are presented as the mean ± SEM from 25 PBS (pooled from each time point) and 8–12 RSV (18 h and day 4) pooled from two independent experiments or 4–5 individual mice from one (day 8) experiment. Statistical significance of differences was determined by two-way ANOVA with Bonferroni’s post hoc test. Data from (c) are presented as the mean ± SEM of 9 (PBS) or 10 (RSV isotype/α-Ly6G) individual mice pooled from two independent experiments. Each symbol represents an individual mouse. Statistical significance of differences was determined by one-way ANOVA with Tukey’s post hoc test.