Fig. 6. PPIN rewiring and CRC prognosis.

a The top 20 most rewired bait proteins. Interactions where the prey protein was identified only in EGFRNet^mtKRAS-Hi or EGFRNet^mtKRAS-Lo are shown as solid red or blue lines, respectively. Rewired bait-prey interactions where prey abundance was significantly higher in EGFRNet^mtKRAS-Hi or EGFRNet^mtKRAS-Lo are shown as dotted red or blue lines, respectively. Bait–prey interactions which were not significantly different are gray. b Six hundred and twenty-nine CRC patients from the TCGA were divided into two groups, those with alterations in the top 20 rewired bait proteins (339; 54%) and those without alterations in the top 20 rewired bait proteins (290; 46%). The alterations assessed were mutations, copy number changes, mRNA expression changes, and protein expression changes. Kaplan–Meier survival curves were plotted for the two patient groups using PRISM 7.0.3. Five-year survival was 53.5% for patients with genetic alterations affecting the top 20 rewired nodes compared to 68.5% for patients without alterations in these proteins, and ten-year survival was 34.61 vs. 61.43%, respectively. The log-rank test was used to assess statistical significance. c The bottom 20 least rewired bait proteins. d There was no significant (NS) difference in survival between patients with alterations in the bottom 20 least rewired bait proteins and those without. Source data are provided as a Source Data file.