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. 2020 Jan 24;11:513. doi: 10.1038/s41467-019-14258-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Expression of Erdr1 in intestinal small and large organoids derived from SPF, exGF and GF mice. n = 4. b ChIP analysis for histone H3 acetylation (H3Ac) in the Erdr1 promoter region using large intestine (LI) organoids derived from SPF, exGF, and GF mice. n = 4; two independent experiments. c Representative images of LI organoids derived from SPF, exGF, and GF mice and cultured ±Erdr1 (1 μg) for 6 days. d Organoid efficiency of LI organoids cultured ±Erdr1. n = 4; representative of three experiments. e Frequency of budding organoids cultured ±Erdr1 for 6 days. n = 4; representative of three experiments. f Surface area of LI organoids cultured ±Erdr1. n = 21; representative of three experiments. All data are presented as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001 by unpaired, two-tailed t-tests.