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. Author manuscript; available in PMC: 2020 Jan 25.
Published in final edited form as: Cell Rep. 2019 Nov 5;29(6):1594–1609.e5. doi: 10.1016/j.celrep.2019.09.078

Figure 6. Knockdown of hnRNP M Enhances a Macrophage’s Ability to Control Viral Infection.

Figure 6.

(A) Viral replication in hnRNP M KD and SCR control RAW 264.7 macrophages infected with VSV (MOI = 1.0, MOI = 0.1, or Mock) at 2, 4, and 8 h post-infection.

(B) qRT-PCR of Ifnb mRNA levels in SCR control and hnRNP M KD cells at 2 and 4 h post-infection, MOI = 1.

(C) qRT-PCR of Mx1 transcript in VSV-infected SCR control and hnRNP M KD cells at 2, 4, and 8 h post-infection, MOI = 1.

(D) qRT-PCR of IL6 transcript in SCR control and hnRNP M KD cells at 2, 4, and 8 h post-infection MOI = 1.

(E) Differential gene expression in hnRNP M KD cells compared to SCR control macrophages from earlier RNA-seq analysis (Figure 1) highlighting known viral response genes.

(F) qRT-PCR of Ifit, Irf7, Rsad2, and Gbp5 mRNA levels in SCR control and hnRNP M KD cells at 4 h post-infection, MOI = 1.

(A)-(E) are representative of 2 biological replicates with values indicating means ± SEM, n = 2. (F) is representative of 2 independent experiments that showed the same result with values representing means (SD), n = 3.