Fig. 3.

Nonsense/frameshift mutations caused exon skipping by H492-dys plasmid. a Schematic representation of H492 minigene constructs consisting of DMD gene region encompassing exon harboring a mutation (Dys-Exon) with flanking introns (200 bp each) and two artificial hybrid exons (exon A and B). b–j The RT-PCR products from HeLa cells transfected with wild-type or mutant H492 minigene were separated on agarose gel. The structures of each PCR product are shown at the right of the panels. The longer PCR products were included inserted exon and smaller one did not contain them without any cryptic splicing. *Primer dimer