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. 2019 Sep 25;13(1):17–26. doi: 10.1007/s12195-019-00601-3

Figure 2.

Figure 2

Confirmed overexpression of NHE3 and AQP1. (a) western blot analysis confirming cumate-inducible NHE3 expression (detected by anti-HA and anti-NHE3 antibodies) and AQP1 expression (via anti-flag and anti-AQP1 antibodies). β-actin is provided as a loading control. Molecular weight (MW) markers are labeled to the left of the blots. (b) Immunofluorescent microscopy of transfected cells. Immunofluorescent microscopy of stably transfected MDCK cells expressing cumate-inducible NHE3 expression (detected by anti-HA and anti-NHE3 antibodies) and AQP1 expression (via anti-flag and anti-myc antibodies). Dapi indicates nuclear staining.