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. 2019 Sep 25;13(1):17–26. doi: 10.1007/s12195-019-00601-3

Figure 4.

Figure 4

Confirmation of cells surface delivery of NHE3 and AQP1. Cell surface biotinylation was used to confirm surface delivery of NHE3 and AQP in double stably-transfected clones. FT flow through, IBP isolated biotinylated proteins. Western detection was used to evaluate surface expression. Molecular weight (MW) marker weights are indicated on the blots. WT, wild type cells; 55, clone number 55; 60, clone number 60. Anti-HA antibodies were used for NHE3 detection and anti-flag antibodies were used for AQP1 detection.