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. 2019 Sep 4;13(1):99–112. doi: 10.1007/s12195-019-00597-w

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Cytotoxicity of HeLa cells treating with various microbubbles and ultrasonic treatment. (a) Cell viability of HeLa cells treating with NMBs, CMBs, and SCMBs4 with or without ultrasonic treatment by CCK-8 assay (n = 5). **p < 0.01 compared with corresponding group without ultrasonic treatment, ^##p < 0.01 compared with CMBs + U group. (b) Fluorescent images of SCMBs4 targeted to HeLa cells by confocal laser scanning microscopy. (b1) Bright-field microscopy image for SCMBs4 (with anti-ICAM-1 free) treating. (b2) Fluorescent image by exciting at 488 nm for SCMBs4 (with anti-ICAM-1 free) treating. (b3) Bright-field microscopy image for anti-ICAM-1 SCMBs4 treating. (b4) Fluorescent image by exciting at 488 nm for anti-ICAM-1 SCMBs4 treating. Scale bar: 50 μm.