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. 2019 Oct 17;13(1):45–60. doi: 10.1007/s12195-019-00602-2

Figure 3.

Figure 3

Paracrine mouse CCN4 promotes mouse and human melanoma cell migration and invasion. (a) Mouse CCN4 secretion from knock-out, control, and over-expression cells derived from mouse fibroblast NIH3T3 cells. Culture media were tested by ELISA after cells were plated in serum-free medium for 48 h. (b) Migration comparison of B16F10 and B16F10-KO cells. Transwell migration assays were performed in triplicates and serum-free conditioned media from indicated cells was used. (c) Invasion comparison of B16F10 and B16F10-KO cells. Transwell invasion assays were performed in triplicates and serum-free conditioned media from indicated cells was used. (d) Cell invasion assays with indicated chemoattractant (conditioned media) using human metastatic melanoma cell lines (RPMI-7951, SK-MEL-3, SH-4 and SK-MEL-24). *p value < 0.05; **p value < 0.01; ***p value < 0.001; and ns: not significant.