. 2020 Jan 1;17(1):298. doi: 10.3390/ijerph17010298

Table 2.

The description and promoter methylation percentages (Met%) of four candidate genes with relatively differential methylation identified by methylation-dependent fragment separation (N = 22).

Gene (ID)	CpG Island Location	Gene Function	Map
Gene (ID)	TSS Position	Promoter Methylation Percentage (Met%) (mean ± SD) upon Low and High BPA Exposure	Map
AR (367)	ChrX: 66763684–66764077	Development and maintenance of the male sexual phenotype, DNA-binding transcription factor that regulates gene expression	NM_000044 Genome Position: chX 66680589–66860844(+)
AR (367)	66763873	Bottom-low vs. top-high exposure 37.76 ± 23.87 vs. 23.73 ± 18.33 p = 0.138	NM_000044 Genome Position: chX 66680589–66860844(+)
TNFα (7124)	Chr6: 31543344–31544344	Pro-inflammatory cytokine- stimulates the acute phase reaction and airway inflammation and regulates immune cells	NM_000594 Genome Position: chr6 31651328–31654089(+)
TNFα (7124)	31543350	Bottom-low vs. top-high exposure 42.15 ± 36.60 vs. 23.20 ± 22.37 p = 0.16	NM_000594 Genome Position: chr6 31651328–31654089(+)
IL-4 (16189)	Chr5: 132035956–132036176	Activates B-cell and T-cell proliferation induces B-cell class switching to IgE	NM_000589 Genome Position: chr5 132037271–132046267(+)
IL-4 (16189)	132040541 Specific primers for IL-4 gene as below: Forward 5′-GTTGATTGGTTTTAAGTGATTGATAATT-3’ and backward 5′-Biotinylated ATACCCAAATAAATACTCACCTTTCACT-3’.	Bottom-low vs. top-high exposure 89.36 ± 7.65 vs. 85.73 ± 6.99 p = 0.26	NM_000589 Genome Position: chr5 132037271–132046267(+)
MAPK1 (5594)	Chr22: 20443948–20551970	Mediates cell growth, adhesion, survival, and differentiation. Regulates meiosis, mitosis and postmitotic functions	NM_002745 Genome Position: chr22 2044394–20551970(-)
MAPK1 (5594)	20447613	Bottom-low vs. top-high exposure 79.82 ± 5.56 vs. 69.82 ± 5.88 p = 0.001	NM_002745 Genome Position: chr22 2044394–20551970(-)

Figure source: MethPrimer 2.0.