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. 2019 Dec 29;21(1):238. doi: 10.3390/ijms21010238

Figure 2.

Figure 2

TMP195 enhances drug-induced apoptosis in ABCB1-overexpressing cancer cells and ABCG2-overexpressing cancer cells. Dot plots (upper panel) and quantification (lower panel) of (A) drug-sensitive KB-3-1 cells and the MDR variant KB-V-1 cells treated with either DMSO (control), 10 μM of TMP195 (+TMP195), 500 nM of colchicine (+colchicine), or a combination of 500 nM of colchicine and 10 μM of TMP195 (+colchicine +TMP195), and (B) drug-sensitive S1 and the MDR variant S1-M1-80 cells treated with either DMSO (control), 10 μM of TMP195 (+TMP195), 5 μM of topotecan (+topotecan) or a combination of 5 μM of topotecan and 10 μM of TMP195 (+topotecan +TMP195). Cells were treated with respective regimens, isolated, and analyzed by flow cytometry as described previously [30]. Representative dot plots and quantifications of apoptotic cell populations are presented as mean ± SD calculated from at least three independent experiments. ** p < 0.05; ** p < 0.01; *** p < 0.001, versus the same treatment in the absence of TMP195.