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. 2019 Dec 31;21(1):274. doi: 10.3390/ijms21010274

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Immunostaining analysis of kidney sections using P53 (red) and Zonula Occludens 1 (ZO1) (green) antibodies and DAPI (blue) showed increased P53 staining in the ADR- and nephrotoxic serum (NTS)-injured mice. Scale bar = 10 µm. (B) Quantitative analysis of mean pixel intensity showed increased P53 expression in the glomeruli of ADR- and NTS-injured mice compared to the control. A total of 30–40 glomeruli from three mice representing each group were used for statistical analyses. Data are presented as mean ± SEM; two-tailed t-test; *** p ≤ 0.001 ADR vs. control and NTS vs. control.