Figure 5.

Analysis of the cleavage specificity of platypus DDN1-like (also a chymase) by the use of recombinant protein substrates. The different panels show the cleavage of a number of substrates by the platypus DDN1-like. The sequences of the different substrates are indicated above the pictures of the gels. The red residues highlight the major difference between this substrate and the reference substrate positioned at the beginning (Left side) of this panel. The time of cleavage, in minutes, is also indicated above the corresponding lanes of the different gels. The un-cleaved substrates have a molecular weight of approximately 25 kDa and the cleaved substrates appear as two closely-located bands with a size of 12–13 kDa.