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. 2019 Dec 21;21(1):89. doi: 10.3390/ijms21010089

Figure 2.

Figure 2

Figure 2

L7™ TFO2 hPSC xeno-free media supports the microcarrier (MC)-based expansion of RTiPSC3B and RTiPSC4i human-induced pluripotent stem cells (hiPSCs) leading to >2 × 106 cells/mL (A) and >10 fold expansion (B) in 17 days. Small- and large-sized MCs support the cell growth (C) and expansion (D) of RTiPSC3B in L7™ TFO2 hPSC xeno-free media. Inoculating less cells/mL does not compromise cell yield, but leads to rapid cell growth and higher cell expansion of RTiPSC4i (E,F) and RTiPSC3B (G,H), which were cultured on small- and large-sized MCs. Coating MCs with the L7™ hPSC Matrix is essential for the attachment and expansion of hiPSCs in L7™ TFO2 hPSC medium. RTiPSC3B inoculated at 0.2 × 106 cells/mL with small-sized microcarriers (I) and LiPSC18R inoculated at 0.04 × 106 cells/mL with large-sized microcarriers (J). RTiPSC4i inoculated at 0.04 × 106 cells/mL with large-sized coated and uncoated microcarriers. Cell growth (K) and fold expansion (L) are shown.