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MiR-204 antagomir enhances the expression of osteogenic biomarkers in AVICs from normal aortic valves. AVICs from normal human aortic valves were treated with miR-204 antagomir (50 nM) or irrelevant oligonucleotide (miR-C, 50 nM) for 72 h. Representative immunoblots and densitometric data show that miR-204 antagomir up-regulates the levels of Runx2, Osx and ALP. Mean ± SE; n = 6 distinct cell isolates; * p < 0.05 vs. untreated control; † p < 0.05 vs. cells treated with miR-C.
