AEX	Anion-exchange chromatography
Ara-C	Cytosine-β-D-arabinofuranoside
BoNT	Botulinum neurotoxin
BoNT/D	Botulinum neurotoxin D
BOTOX	Botulinum toxin A complex
CGRP	Calcitonin gene-related peptide
CGRP8-37	CGRP receptor antagonist
CS	C-terminal sortase motif
DC	Di-chain
/DΔHC	BoNT/D lacking the neuronal binding domain HC
/D-CGRP8-37	/DΔHC-CS and CGRP8-37 ligated conjugate
/DIL-1β	/DΔHC-CS and IL- 1β ligated conjugate
DMEM	Dulbecco’s modified Eagle’s medium
DPBS	Dulbecco’s phosphate-buffered saline
DRGs	Dorsal root ganglion neurons
DTT	Dithiothreitol
ECL	Enhanced chemiluminescence reagent
EIA	Enzyme immunoassay
FBS	Fetal bovine serum
H6	His6 tag
HC	Heavy chain domain
HC	C-terminal half of HC
HK	High potassium
HN	N-terminal half of HC
IFNγ	Interferon gamma
IL-1β	Interleukin 1β
LC	Light chain domain
LK	Low potassium
LPS	Lipopolysaccharides
NGF-2.5S	Nerve growth factor 2.5S
NSAIDs	Nonsteroidal anti-inflammatory drugs
RA	Rheumatoid arthritis
SC	Single-chain
SNAP-25	Synaptosomal-associated protein 25k
SNAREs	Soluble N-ethylmaleimide-sensitive factor attachment protein receptors
TNFα	Tumor necrosis factor-α
Trx	Thioredoxin tag
Trx-H6	Trx-His6 tag
Trx-H6-IL-1β	Thioredoxin-His6-Gly5-IL-1β protein
US	Unstimulated
VAMP	Vesicle associated membrane protein