. 2020 Jan 6;17(1):376. doi: 10.3390/ijerph17010376

Table 3.

Primers Used in This Study and qPCR Performance.

Target Gene	Primer	Sequence (5′-3′)	Annealing Temp (°C)	Length (bp)	qPCR Performance		Reference
Target Gene	Primer	Sequence (5′-3′)	Annealing Temp (°C)	Length (bp)	R²	Efficiency	Reference
Eubacterial16S rRNA gene ¹	27F	AGAGTTTGATCMTGGCTCAG	60	~1500	--	--	[26]
Eubacterial16S rRNA gene ¹	1492R	GGWTACCTTGTTACGACTT	60	~1500	--	--	[26]
Eubacterial 16S rRNA gene	1369F	CGGTGAATACGTTCYCGG	60	124	0.9966	91.09%	[27]
Eubacterial 16S rRNA gene	1492R	GGWTACCTTGTTACGACTT	60	124	0.9966	91.09%	[27]
PAOs 16S rRNA gene	518F	CCAGCAGCCGCGGTAAT	65	351	0.9985	95.56%	[28]
PAOs 16S rRNA gene	846R	GTTAGCTACGGCACTAAAAGG	65	351	0.9985	95.56%	[28]
Nitrosomonas spp. amoA gene	amoA-1F	GGGGTTTCTACTGGTGGT	60	491	0.9990	91.62%	[29]
Nitrosomonas spp. amoA gene	amoA-2R	CCCCTCKGSAAAGCCTTCTTC	60	491	0.9990	91.62%	[29]
Nitrospira spp. 16S rRNA gene	NSR1113F	CCTGCTTTCAGTTGCTACCG	60	150	0.9994	93.82%	[30]
Nitrospira spp. 16S rRNA gene	NSR1264R	GTTTGCAGCGCTTTGTACCG	60	150	0.9994	93.82%	[30]
Nitrobacter spp. 16S rRNA gene	Nitro119F	ACCCCTAGCAAATCTCAAAAAACCG	60	227	0.9994	92.40%	[31]
Nitrobacter spp. 16S rRNA gene	Nitro1423R	CTTCACCCCAGTCGCTGACC	60	227	0.9994	92.40%	[31]

¹ Used in conventional PCR to generate nearly complete 16S rRNA gene for cloning.