Figure 7.

In vitro tests of C1 hydrogels. (a) Percentage weight ratio of C1 hydrogel immersed in culture medium at 37 °C, up to 7 days (i.e., 168 h). (b) Percentage L929 cell viability of cell cultured in culture medium eluates in contact with C1 for 1, 3 and 7 days, compared to cells cultured in culture medium (i.e., control). (c,d) In vitro direct cytocompatibility tests of cells embedded in C1, in alginate/Ca²⁺ hydrogel (i.e., hydrogel control) and seeded on tissue culture plastic (i.e., tissue culture plastic (TCPS) as control): (c) percentage of viable L929 cells (* p < 0.05, comparing a sample to the others at the same time point) and (d) representative LIVE/DEAD staining images (scale bar: 100 µm).