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. 2019 Dec 20;21(1):60. doi: 10.3390/ijms21010060

Figure 2.

Figure 2

Expression profiling of DRG2 in the mouse brain. (A) Western blot analysis for DRG2 protein tissue distribution. (B) Western blot analysis for DRG2 expression in mouse brain regions. (C) Changes in DRG2 protein expression with age in the four brain regions such as CTX, cerebral cortex; HIP, hippocampus; CB, cerebellum and WB, whole brain. DRG2 expression was normalized to actin. Each data point represents the mean ±SEM (n = 6). (D) In situ hybridization analysis of DRG2 expression in the mouse brain. Sagittal sections of adult mouse brain were hybridized with DRG2 antisense probes. Representative images for DRG2 mRNA (red dots) and nuclei counterstained with DAPI (blue). The boxed regions were viewed at higher magnification in the bottom panels. Scale bar 1 mm. (E) Immunohistochemical analysis of DRG2 expression in the sagittal and coronal sections of mouse adult brain using anti-DRG2 primary antibody and Alexa-488-labeled secondary antibodies. Nuclei were stained with DAPI. (a) Sagittal sections of mouse brain. (b–i) Higher magnification of specific regions within sagittal image in (a): (b) CTX, (c) THs, thalamus (d) PB, parabrachial nucleus (e) HY, hypothalamus (f) CB, (g) CTX, (h) HIP, and (i) SN. Arrow in (f) indicates the purkinje cell layer. Scale bar, 100 μm. (j–l) Coronal sections of mouse brain at three different planes. Sagittal diagrams of the brain showing the plane of section were indicated within each images. AOB, accessory olfactory bulb; AUD, auditory areas; BMA, basomedial amygdala nuclear; CA1, cornu ammonis1; CA3, Cornu Ammonis3; DG, dentate gyrus; CB, cerebellum; CP, caudate putamen; CTX, cerebral cortex; ENT, Entorhinal area; HIP, hippocampus; HY, hypothalamus; MB, mid brain; Mo, somatomotor area; NAc, nucleus accumbens; PB, parabrachial nucleus; Pir, piriform cortex; PTLP, posterior parietal association areas; RSP, retrosplenial area; SCN, suprachiasmatic nucleus; SN, substantia nigra; SNc, substantia nigra pars compacta; SNr, substantia pars reticulate; SS, somatosensory area; Str, striatum; SUB, subiculum; THs, thalamus; VIS, visual area; VTA, ventral tegmental area; WB, whole brain.