Figure 4.

Expression analysis of KPR1 regulated genes by qRT-PCR. (A) qRT-PCR validation of the DEGs revealed by RNA-seq experiments; (B) qRT-PCR analysis of the transcriptional abundances of grain filling related genes in the WT, OxKRP1, crkrp2, and crkrp1/2 mutants. 6 DAP seed cDNA were used as templates for this analysis. DEGs: Differentially Expressed Genes. Error bars indicate SD with biological triplicates (n = 3). Asterisks indicate the significant difference between the WT and transgenic lines, as determined by Student’s t-test analysis: * p < 0.05, ** p < 0.01. Primers used in (A) were listed in Table S1. Primers used in (B) were used according to previous reports [22,23].