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. 2019 Dec 19;21(1):37. doi: 10.3390/ijms21010037

Figure 1.

Figure 1

Reduced expression of dysferlin in immortalized myoblasts obtained from dysferlinopathy patients. Dysferlin expression was determined by immunoblotting of total protein extracts from C25 myoblasts obtained from an unaffected donor, or DYSF2, DYSF3, AB320, or ER myoblasts obtained from dysferlinopathy patients. (a) Example of immunoblot detection of dysferlin (upper bands) and β-tubulin (loading control; bottom bands). (b) Relative density (dysferlin/β-tubulin ratio). Data are means ± SEM from 5 independent immunoblots. * p < 0.05 compared to C25 myoblasts (t-test).